THE STUDY OF POSSIBILITY OF BIOLOGICALLY ACTIVE AGENTS SORPTION FROM CULTURE LIQUID ON THE EXAMPLE OF COPROPORPHYRIN III

Abstract

At release of biologically active agents received by means of microbiological synthesis one of
the first stages is filtration of culture liquid with native solution receiving. This stage is especially
labor-consuming if a producer of target substance is the bacterial cell with the size about 1 micron
as insoluble residues of nutrient medium and metabolites of a producer and also cells clog the filtering
surface, thereby increasing hydrodynamic drag force so that filtration practically stops. Various
expensive methods, for example, regular updating of a filter blanket by mechanical removal
of the top part of a filter blanket together with solid fraction of culture liquid are used to overcome
this problem. Such method is applied on drum filters. Drum filters are of large size, take a lot of
place at the plant and are expensive producing and operating. The present article considers the
possibility of filtration stage replacement with more technological process of sorption of a main
product immediately from cultural liquid, using the example of coproporphyrin III a producer of
which is Arthrobacter globiformis bacterium with cell sizes of about 1 micron. The technology of
coproporphyrin III extracting and its purification with coproporphyrin III sorption immediately
from culture liquid without biomass separation in pulsed fluidized mode with the subsequent desorption
was developed. Further stages of extracting and purification include settling of target substance
by means of the solution рН value change, porphyrine extraction from the precipitation,
chromatographic purification on a molecular sorbent, the transfering of coproporphyrin III to potassium
salt and drying. The technology was tested in vitro and the coproporphyrin III with high
degree of cleaning was obtained.